Minitube cryoguard media androhep plus#

Today, Minitube's product portfolio consists of all the necessary machinery, equipment and consumables to meet the individual needs of large, medium sized and small boar stud operations. Another milestones in porcine AI technology development were the world’s first long-term extender for boar semen, Androhep®, in 1988 or the first fully automatic boar semen packaging (BSP) line worldwide only one year later. Simmet’s enthusiasm, one of the first boar studs in Germany was established soon after.Īs a pioneer in the field of artificial insemination, Minitube has provided expertise and high quality products to the boar stud and sow farm for five decades.Ĭare for an example? In 1972, with the Spirette®, Minitube developed the world’s first disposable AI catheter for pig insemination. Adjust the sperm concentration to 510 × 10 8 /mL by adding Androhep CryoGuard Cooling Extender.

For freshly collected semen, dilute 1:10,000 to count. Calculate the sperm concentration by using a hemocytometer. Thanks to the foresight of the management at the lower Bavarian farmer's cooperative and to Dr. Remove supernatant and add 5 mL of Androhep CryoGuard Cooling Extender and mix semen well. He wrote that he was “…immediately convinced that AI in pigs could offer farmers in Germany tremendous and immediate advantages.” In the same year, he and his wife Irmingard returned to Sweden to learn all they could about AI in swine. Ludwig Simmet went to Sweden in 1960 and was introduced to the AI techniques then being used in swine. However, Minitube did not play any less of a role in developing porcine reproduction technology.ĭr. All rights reserved.The founding of Minitube in 1970 was closely related to automation of bovine semen processing with a novel straw technology. It is concluded different combinations of commercial freezing extenders and thawing solutions have effects on the quality of cryopreserved boar semen in vitro.Ĭryopreservation Fertility Spermatozoa Swine Viability.Ĭopyright © 2021 Elsevier B.V. The use of T2, as compared with T1 thawing extender, resulted in an enhanced integrity of the plasma and acrosomal membranes (P = 0.008). Place the sperm suspension inside a cooling machine and allow the sperm to cool to 4 C over a 1.5 h period.

Minitube cryoguard media androhep plus#

The sperm plasma and acrosomal membrane integrity (AIMI) were greater (P = 0.009) when samples were preserved in F1 compared to F2 extender. Remove supernatant and add 5 mL of Androhep CryoGuard Cooling Extender and mix semen well. This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard - F1 or Androstar CryoPlus - F2) and thawing (Minitube Cryoguard Thawing solution - T1 or Androstar Plus - T2) extenders. Within 30 min of thawing, intra-cervical insemination was performed using standard spirette or foam tipped AI catheters (Minitube of America, Verona, WI) in the presence of a boar. Sperm thawed in T1 had a greater TMOT (P = 0.008) and PMOT (P = 0.033) at all times evaluated. The thawed sperm were diluted with 80 mL of Androhep CryoGuard Thaw extender (Minitube of America, Verona, WI) in a 26 C waterbath. The sperm progressive motility (PMOT) as time post-thawing increased was greater (P = 0.015) when dilutions occurred using F1 compared with F2 extender. There was no interaction between F × T × Time (P > 0.05), and no interaction between F × T (P > 0.05).

The integrity assessments of the plasma and acrosomal membranes were performed at 30 and 360 min after thawing. The sperm in diluted semen were evaluated for motility kinetics at 30, 180, and 360 min after thawing. Four straws from each treatment sample were thawed and diluted in T1 or T2, resulting in four treatments (F1-T1, F1-T2, F2-T1, and F2-T2). Samples were aliquoted in cryopreservation extender F1 or F2. Ejaculates were collected, diluted (1:1), and cooled before shipping at 17 ☌ overnight. This study was conducted to evaluate whether there were differences in viability of cryopreserved semen when using two different freezing (Minitube Cryoguard - F1 or Androstar® CryoPlus - F2) and thawing (Minitube Cryoguard Thawing solution - T1 or Androstar® Plus - T2) extenders.